中药药理与临床

目的:考察桂枝对大鼠中枢神经系统的影响。方法:本实验根据中医临床用药习惯,按照桂枝生药量计分设立5、12、30g/kg的桂枝给药组,并设立生理盐水空白对照组和桂皮醛24mg/kg阳性对照组。采用行为药理学实验方法,通过高架十字迷宫实验和旷场实验观察不同剂量桂枝对大鼠一般行为、自主活动及探索活动的影响;通过戊巴比妥钠诱导睡眠实验观察不同剂量桂枝对大鼠睡眠潜伏期和睡眠持续时间的影响。结果:相比生理盐水组,桂皮醛与不同剂量桂枝水煎液灌胃给药后,大鼠外观行为表现为自主活动减少,修饰及嗅探次数增加。随着桂枝给药剂量的增加,大鼠进入高架十字桥开臂的次数和时间明显增加;大鼠进入闭臂的次数明显降低,其中,桂枝12、30g/kg剂量组与生理盐水组相比差异有统计学意义。桂枝12、30g/kg剂量组大鼠在旷场中心区域的停留时间和比例明显增加;大鼠的跨格次数和站立次数明显增加,其中,桂枝30g/kg剂量组与生理盐水对照组相比差异具有统计学意义;桂枝5、12、30 g/kg剂量组大鼠总的运动距离明显降低。以上3个剂量组的桂枝水煎液可增强戊巴比妥钠诱导的催眠作用。结论:本实验研究表明桂枝对大鼠中枢神经系统具有镇静和抗焦虑作用,随着用药剂量的增加镇静作用增强。

Objective: To study the possible role of Gui Zhi on the central nervous system. Method: The battery of ethological tests( open field,elevated plus-maze) was used to obtain information about anxiolytic and antidepressant activity of the Gui Zhi decoctions. The substances were administered 1 hour before the tests at doses of 5,12 and 30 g /kg. Results: In the behavioral tests,Gui Zhi decocotions were found to exert anxiolytic activity in elevated plus maze with no affection of locomotor activity. The different doses of Gui Zhi decocotions increased the time spent in the open arm of the elevated plus-maze. Similar findings were observed also in the central area of open field. With the increase of the dose,the number( P < 0. 05) and time( P < 0. 01) of entries into the open arm of elevated plus maze significantly increased. Number of entries into the closed arms decreased significantly. Compared with the control group,12,30 g /kg dose group was statistically significant difference( P < 0. 05). The residence time and the proportion in the central area of the open field of 12,30 g /kg dose group was significantly increased( P < 0. 05). The number of rats crossing and grooming increased significantly. Compared with the control group,the difference was statistically significant( P < 0. 05). The total distance traveled in Gui Zhi 5,12,30 g /kg dose group were significantly lower Compared with the control group( P < 0. 05). Also,Gui Zhi decocotions could increase the hypnotic action induced by pentobarbital sodium,although these results were not statistically significant. Conclusion: From the results of our study it is evident that Gui Zhi decocotions acted on the CNS. In the highest dose,Gui Zhi decocotion was found to possess rather sedative than anxiolytic or antidepressant activity.