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目的:研究肉桂挥发油化学成分组成及其体外抗氧化、抑菌和抗肿瘤等生物活性。方法:分别利用气相色谱-质谱联用(GC-MS)法和傅里叶红外光谱技术鉴定肉桂挥发油主要活性成分和官能团；紫外分光光度计测定肉桂挥发油总酚含量、DPPH自由基清除活性和β-胡萝卜素漂白抑制率；通过打孔法、微量稀释法分别测定抑菌圈直径、最小抑菌浓度(MIC)和最低杀菌浓度(MBC),考察肉桂挥发油对常见的8种致病菌和6种耐药菌的抑菌活性；MTT法测定肉桂挥发油对不同肿瘤细胞的IC50,DCFH-DA探针法检测细胞内活性氧含量。结果:肉桂挥发油共鉴定出19种化合物，总酚含量为(29.9±1.2) mg GAE/g EO,具有较高的自由基清除能力[DPPH IC50=(4.0±0.4)μg/mL],肉桂挥发油1.6 mg/mL时对β-胡萝卜素漂白的抑制率达68.6%;肉桂挥发油对8种常见细菌及6种耐药菌种均显示出一定的抑制作用；与空白对照组相比，肉桂挥发油对MDA-MB-231乳腺癌细胞显示出显著降低增殖、迁移、侵袭能力，显著诱导MDA-MB-231细胞凋亡(P<0.01)。肉桂挥发油显著上调细胞内ROS水平，上调凋亡相关蛋白Caspase-3和AIF的表达(P<0.01);引起线粒体膜电位下降，促进了细胞凋亡。结论:肉桂挥发油中成分复杂，含有多种活性成分，在抗氧化、抑菌和抗肿瘤等方面具有良好的作用。
Abstract：[1]国家药典委员会.中华人名共和国药典(一部)[S].北京：中国医药科技出版社，2020:142.
[2]侯小涛，郝二伟，秦健峰，等.肉桂的化学成分、药理作用及质量标志物(Q-marker)的预测分析[J].中草药，2018,49(1):20-34.
[3]蒋艳荣，张振海，胡绍英，等.胶态二氧化硅SYLOID244FP固化肉桂挥发油的研究[J].中国中药杂志，2013,38(1):53-56.
[4]高凯，刘香军，刘霞，等.不同壁材丁香精油微胶囊保鲜剂性能研究[J].食品工业，2015,36(2):58-61.
[5]Laura E H,Carmen G,Matthew T,et al.Characterization of beta-cyclodextrin inclusion complexes containing essential oils(trans-cinnamaldehyde,eugenol,cinnamon bark,and clove budextracts) for antimicrobial delivery applications[J].LWT-Food SciTechnol,2013,51(1) :86-93.
[6]钟赣生.中药学[M].中国中医药出版社，2012:216-217.
[7]陈旭，刘畅，马宁辉，等.肉桂的化学成分、药理作用及综合应用研究进展[J].中国药房，2018,29(18):2581-2584.
[8]高铭哲，李婷，田晨琪，等.肉桂化学成分与药理作用研究进展[J].亚太传统医药，2021,17(11):201-205.
[9]黄炜忠，毛鑫，翟锡斌，等.肉桂油的化学成分分析及风险性研究[J].广东化工，2020,47(12):35-37,66.
[10]陈珏锡，张俊丰，李源栋，等.无溶剂微波萃取肉桂精油及成分分析[J].现代食品科技，2021,37(8):258-265,167.
[11]Netopilova M,Houdkova M,Urbanova K,et al.In vitro antimicrobial combinatory effect of Cinnamomum cassia essential oil with 8-hydroxyquinoline against Staphylococcus aureus in liquid and vapour phase[J].J Appl Microbiol,2020,129(4):906-915.
[12]Sufriadi E,Meilina H,Munawar A A,et al.Fourier Transformed Infrared (FTIR) spectroscopy analysis of patchouli essential oils based on different geographical area in Aceh[J].IOP Conference Series:Materials Science and Engineering,2021,1087(1):012067.
[13]HOU Yizhe,LIAN Yunlan,WU Honggen,et al.Quality control of Notopterygii rhizoma et radix using near infrared spectroscopy and chemometrics[J].Vibrational Spectroscopy,2020,111:103181.
[14]Derya E.Carbonic anhydrase enzyme inhibition and biological activities of Satureja hortensis L.essential oil[J].Industrial Crops &Products,2020,156:112849.
[15]Sp?nu S ,Or?an A ,Ionescu D,et al.Comparison of Total Phenolic Content,Antioxidant Activity and Extraction Yield from Apium Graveolens Waste Using Unconventional Extraction Methods[J].IOP Conference Series:Materials Science and Engineering,2020,877(1):012058.
[16]Gyesi J N,Opoku R,Borquaye L S.Chemical Composition,Total Phenolic Content,and Antioxidant Activities of the Essential Oils of the Leaves and Fruit Pulp of Annona muricata L.(Soursop) from Ghana[J].Biochem Res Int,2019,2019:4164576.
[17]DUAN Yishan,Kim Han Soo,Kim GyeongHwuii.Evaluation of Antioxidative activity of Korean Yam (Dioscorea batatas DECNE.) by n-Butanol and Ethyl Acetate Extracts[J].Journal of the Korean Applied Science and Technology,2015,32(2):312-319.
[18]孙长花，丁娟芳，王君，等.迷迭香提取液的抑菌作用及稳定性研究[J].中国调味品，2020,45(11):42-45.
[19]ZHANG Ying,CHENG Risheng,XU Zijie,et al.A special aromatic Chrysanthemum breed with high content of thujone[J].Pharmacognosy Magazine,2020,16(71):625-629.
[20]Behrooz A B,Fereshteh F,Fahimeh L A,et al.Chemical Composition and Antioxidant,Antimicrobial,and Antiproliferative Activities of Cinnamomum zeylanicum Bark Essential Oil[J].Evidence-Based Complementary and Alternative Medicine,2020,2020:5190603.
[21]何翠薇，谢艳林，欧庆勇.傅里叶变换红外光谱法鉴别肉桂药材模型研究[J].时珍国医国药，2013,24(10):2430-2431.
[22]YANG HsinLing,Thiyagarajan V,Shen P C,et al.Anti-EMT properties of CoQ0 attributed to PI3K/AKT/NFKB/MMP-9 signaling pathway through ROS-mediated apoptosis[J].J Exp Clin Cancer Res,2019,38(1):186.
[23]易健，舒徐.活性氧对细胞凋亡和增殖的调控作用[J].基础医学与临床，2013,33(10):1127-1130.
[24]WANG H C R,Choudhary S.Reactive oxygen species-mediated therapeutic control of bladder cancer [J].Nat Rev Urol,2011,8:608-616.
基本信息:
DOI：10.13412/j.cnki.zyyl.20230719.002
中图分类号:R284.1;R285.5
引用信息:
[1]廖正根,温佳豪,徐希强,等.肉桂挥发油化学成分及其体外抗菌、抗氧化、抗肿瘤生物活性研究[J].中药药理与临床,2024,40(01):69-76.DOI:10.13412/j.cnki.zyyl.20230719.002.
基金信息:
江西中医药大学中药制剂技术与装备创新团队(编号：CXTD22006);江西中医药大学校级大学生创新创业训练计划项目(编号：202210412365、202210412400);江西中医药大学校级研究生创新专项资金项目(编号：JZYC21S38)
2023-07-19
2023-07-19
2023-07-19