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目的:基于网络药理学研究清肺排毒汤在治疗新型冠状病毒肺炎(Corona Virus Disease 2019,COVID-19)的作用机制。方法:使用TCMSP数据库对清肺排毒汤的中药活性成分进行检索、筛选;运用Gene Gards数据库对疾病靶点进行预测、筛选,构建活性成分-靶点网络图。将得到的靶点利用STRING进行蛋白互作(protein-protein interaction,PPI)网络构建,并进行基因本体(gene ontology,GO)和KEGG富集分析,构建靶点-信号通路网络图。结果:本文显示共筛选到217个中药成分靶点,新型冠状肺炎(COVID-19) 200个相关靶点,从而得到药物-疾病的共同靶点51个,富集于生物过程及30条信号通路上,如TNF信号通路、IL-17信号通路、NF-κB信号通路及Th17细胞分化等。结论:本研究预测出清肺排毒汤治疗COVID-19的前五个主要活性成分是槲皮素、木犀草素、山奈酚、柚皮素、异鼠李碱;其主要是通过调节MAPK1、MAPK3、MAPK8、MAPK14、IL-6、RELA、STAT1等靶点,调控TNF、NF-κB等信号通路来抑制炎症反应、调节免疫功能,减轻肺损伤,保护神经功能,从而达到治疗COVID-19的目的。
Abstract：Objective: To study Qingfeipaidu decoction and its mechanism in the treatment of COVID-19 based on network pharmacology. Methods: TCMSP database was used to search and screen the active components of Qingfeipaidu decoction,and Gene Gards database was used to predict and screen the disease target,the active component-target network diagram was established. STRING was used to construct proteinprotein interaction( PPI) network. Gene Ontology( Go) and KEGG enrichment analysis were performed,the target signal pathway network map was drawn. Results: A total of 217 targets of traditional Chinese medicine and 200 targets of COVID-19 were screened,then 51 common drug-disease targets were obtained,mostly enriched in biological processes and 30 signaling pathways,such as TNF signaling pathway,IL-17 signaling pathway,NF-κB signaling pathway and Th17 cell differentiation. Conclusion: The five main active components of Qingfeipaidu decoction in the treatment of COVID-19 are quercetin,luteolin,kaempferol,naringin and isorhamnetine. They can inhibit inflammatory reaction,regulate immune function,reduce lung injury and protect nerve function by regulating targets to achieve the purpose of treating COVID-19,such as mapk1,mapk3,mapk8,Mapk14,IL6,RELA and STAT1 and so on.
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基本信息:
DOI：10.13412/j.cnki.zyyl.20200305.001
中图分类号:R285
引用信息:
[1]许冬玉,许玉龙,王至婉,等.基于网络药理学研究清肺排毒汤治疗新型冠状病毒肺炎的作用机制[J].中药药理与临床,2020,36(01):26-32.DOI:10.13412/j.cnki.zyyl.20200305.001.
基金信息:
国家自然科学基金项目(编号:81703946、81973791、61902113);; 河南省高校重点科研项目(编号:20B520012、16A520060、17B520017);; 河南中医药大学博士基金项目(编号:BSJJ2015-19)
2020-03-05
2020-03-05
2020-03-05