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数以亿计的微生物在肠道内构成了肠道微生态系统，该系统在维持人体生理功能，参与营养物质吸收，调节人体健康等方面发挥重要作用。宏基因组学在肠道微生物中的应用可以追溯到20世纪末和21世纪初，其工作集中在测定不同人群之间的微生物组成差异。早期的肠道微生物研究主要依赖于16S rRNA基因测序技术，获得肠道微生物群落的组成，并对其多样性和功能进行初步了解。但该途径无法准确区分微生物的亚种和菌株。目前，通过对大规模宏基因组数据的分析，能够快速、准确地获得肠道微生物群落的遗传信息，并探索其多样性、代谢功能、宿主相互作用、等。本文探讨了宏基因组学技术在肠道菌群研究中的应用，以期为肠道微生物在医学、药学领域的深入研究提供参考。
Abstract：The intestinal microbiota, consisting of billions of microorganisms, plays a crucial role in maintaining human physiological functions, participating in nutrient absorption, and regulating human health. The application of metagenomics in the research on intestinal microbiota can be traced back to the late 20th and early 21st centuries, focusing on comparing the microbial composition among different populations. Early studies in this field primarily employ 16S rRNA gene sequencing to reveal the composition, diversity, and functions of intestinal microbiota. However, this approach cannot accurately distinguish microbial subspecies and strains. The recent studies based on metagenomics can rapidly and accurately obtain the genetic information of intestinal microbiota and explore their diversity, metabolic functions, and interactions with hosts. This article introduces the application of metagenomics in the research on intestinal microbiota, aiming to provide references for in-depth studies of intestinal microbiota in the fields of medicine and pharmacy.
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基本信息:
DOI：10.13412/j.cnki.zyyl.20230726.002
中图分类号:R37
引用信息:
[1]高世勇,魏新瑞,唐博琪,等.基于宏基因组学的肠道微生物研究进展[J].中药药理与临床,2024,40(03):115-119.DOI:10.13412/j.cnki.zyyl.20230726.002.
基金信息:
黑龙江省自然科学基金资助项目(编号：LH2021H002); 哈尔滨商业大学教师“创新”项目支持计划项目(编号：LH2021H002); 中央支持地方高校改革发展基金优秀青年人才项目(编号：2020YQ12); 黑龙江省中医药经典普及化研究专项课题(编号：ZYW2023-074); 黑龙江省卫生计生委科技计划(编号：2017-132); 黑龙江省教育厅面上项目(编号：12541571)
2023-07-26
2023-07-26
2023-07-26