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目的:基于数据挖掘与网络药理学分析期刊与专利文献中治疗动脉粥样硬化疾病方剂的用药配伍规律及作用机制。方法:筛选中国知网、万方、维普数据库、PubMed等数据库中近10年治疗动脉粥样硬化疾病的方剂，整理后采用IBM SPSS Statistics 24、Clementine 12.0等数据挖掘处理软件，分析药物配伍之间的内在联系及关联规则，总结治疗动脉粥样硬化方剂中的用药规律、核心药物配伍以发现新方；并运用中药系统药理学数据(TCMSP)等数据库对其核心药物进行作用机制分析，构建靶基因-信号通路关系网络。结果:通过输入主题词“动脉粥样硬化”、“中药”、“中医”,收集整理近10年有关中医药治疗动脉粥样硬化的文献258篇，收集处方153首，通过频数分析和关联规则分析得到高频药物22个，核心药对19个；对核心药对“红花-桃仁-当归”进行网络药理学分析，前十位靶点分别是SRC、JUN、CASP3、MAOA、SLC6A4、ADRA1B、CHRM2、ADRA2C、PRKCA、BCHE;Reactome通路富集分析主要富集在胺配体结合受体通路、雌激素依赖性基因表达通路、细胞凋亡、血小板聚集(堵塞形成)、白介素4和白介素13信号通路、MECP2神经元受体等信号通路。结论:治疗动脉粥样硬化的方剂多以活血行气、散瘀止痛为主，辅以补血活血、补气益气，其核心药物对配伍活性成分主要通过干扰SRC、JUN、CASP3、MAOA等靶点及调节细胞凋亡、血小板聚集(堵塞形成)、神经元受体及炎症相关信号通路等来治疗动脉粥样硬化。
Abstract：Objective: To analyze the medication regularity and mechanism of Chinese medicinal prescriptions for atherosclerosis in journals and patents based on data mining and network pharmacology. Methods: Prescriptions indicated for atherosclerosis in databases such as CNKI,Wanfang Data, VIP,and PubMed in the past 10 years were screened out. IBM SPSS Statistics 24 and Clementine 12.0 were used to analyze the compatibility of drugs and association rules. The medication regularity and core drug compatibility of the Chinese medicinal prescriptions for atherosclerosis were summarized for the development of new prescriptions. Databases, such as Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP),were used to analyze the underlying mechanism of core drugs and construct a target gene-signaling pathway network. Results: With the terms “atherosclerosis”,“Chinese medicine”,and “traditional Chinese medicine”,258 papers on Chinese medicine in treating atherosclerosis in the past 10 years were obtained, with 153 Chinese medicinal prescriptions involved. Twenty-two high-frequency drugs and 19 core drug combinations were collected by frequency analysis and association rules. The core drug combination, Honghua(红花)-Taoren(桃仁)-Danggui(当归),was analyzed by network pharmacology, and the results revealed that the top 10 target genes were SRC,JUN,CASP3,MAOA,SLC6A4,ADRA1B,CHRM2,ADRA2C,PRKCA,and BCHE. Reactome pathway enrichment analysis indicated that the main pathways included the amine ligand-binding receptor pathway, estrogen-dependent gene expression pathway, apoptosis, platelet aggregation(thrombosis),interleukin IL-4 signaling pathway, IL-13 signaling pathway, and MECP2-regulated neuronal receptor signaling pathway. Conclusion: The Chinese medicinal prescriptions for atherosclerosis are mainly based on Huoxuexingqi(活血行气) and Sanyuzhitong(散瘀止痛),assisted with Buxuehuoxue(补血活血) and Buqiyiqi(补气益气). Its therapeutic effect on atherosclerosis was achieved by interfering with SRC,JUN,CASP3,MAOA,and other target genes, and regulating cell apoptosis, platelet aggregation(thrombosis),neuron receptors, and inflammation-related signaling pathways via core active components.
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基本信息:
DOI：10.13412/j.cnki.zyyl.20211015.004
中图分类号:R285
引用信息:
[1]狄培琰,康乐,孟驿佳,等.基于数据挖掘和网络药理学的中医药治疗动脉粥样硬化用药规律及特点分析[J].中药药理与临床,2023,39(01):85-92.DOI:10.13412/j.cnki.zyyl.20211015.004.
基金信息:
国家中医药管理局标准化项目(编号：GZY-FJS-2020-219); 河南省重大公益专项(编号：201300310100); 建生鲜药创新基金(编号：JSJC-20200101-053); 河南省药品监督管理局科技计划项目(编号：2020DB050-55)
2021-10-15
2021-10-15
2021-10-15