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目的：探讨理肺消积丸经lncRNA HOTAIR/Wnt/β-catenin通路逆转A549细胞上皮间质转化（EMT）抑制NSCLC细胞侵袭的机制。方法：将A549细胞分为模型对照组、HOTAIR过表达组、HOTAIR敲低组、理肺消积含药血清组、HOTAIR过表达+理肺消积含药血清组、HOTAIR敲低+理肺消积含药血清组。分别采用CCK8实验检测细胞活力；划痕实验检测细胞迁移能力；Transwell技术检测细胞侵袭能力；qPCR技术检测lncRNA HOTAIR、Wnt1、β-catenin、Snail、Vimentin和E-cadherin基因表达；Western-blot技术检测Wnt1、β-catenin、Snail、Vimentin和E-cadherin蛋白表达；免疫荧光技术检测Snail、Vimentin和E-cadherin的蛋白表达及定位情况。结果：与模型对照组比较，HOTAIR过表达组细胞活力、迁移及侵袭能力明显提高（P<0.05或P<0.01），Wnt1、β-catenin、Snail、Vimentin基因及蛋白表达显著上调，E-cadherin基因及蛋白表达显著下调（P<0.05或P<0.01），同时HOTAIR过表达可促进Snail和Vimentin胞内积累，并减弱E-cadherin的膜定位，诱导细胞发生EMT；HOTAIR敲低组和理肺消积含药血清组均可逆转上述指标（P<0.05或P<0.01），且两者联合干预效果优于单独作用组（P<0.05或P<0.01）。结论：理肺消积丸可逆转A549细胞上皮间质转化抑制NSCLC侵袭，机制可能与其调控lncRNA HOTAIR/Wnt/β-catenin通路有关。
Abstract：Objective: To investigate the mechanisms by which Lifei Xiaoji (理肺消积) Pills reverse epithelial-mesenchymal transition (EMT) of A549 cells and inhibit NSCLC cell invasion through the lncRNA HOTAIR/Wnt/β-catenin pathway. Methods: A549 cells were divided into the model control group， HOTAIR overexpression group， HOTAIR knockdown group， Lifei Xiaoji drug-containing serum group， HOTAIR overexpression + Lifei Xiaoji drug-containing serum group， and HOTAIR knockdown + Lifei Xiaoji drug-containing serum group. Cell viability was detected using the CCK-8 assay， cell migration ability was assessed using the scratch wound-healing assay， and cell invasion ability was evaluated using the Transwell assay. The expression levels of the HOTAIR， WNT1， CTNNB1， SNAI1， VIM， and CDH1 genes were detected by qPCR. The protein expression levels of Wnt1， β-catenin， Snail， Vimentin， and E-cadherin were detected by Western blot. The protein expression and localization of Snail， Vimentin， and E-cadherin were detected by immunofluorescence staining. Results: Compared with the model control group， the HOTAIR overexpression group showed significantly increased cell viability， migration， and invasion abilities (P < 0.05 or P < 0.01)， significantly upregulated expression of WNT1， CTNNB1， SNAI1， and VIM at both the gene and protein levels， and significantly downregulated expression of E-cadherin at both the gene and protein levels (P < 0.05 or P < 0.01). Moreover， HOTAIR overexpression promoted the intracellular accumulation of Snail and Vimentin and attenuated the membrane localization of E-cadherin， inducing EMT. Both the HOTAIR knockdown group and the Lifei Xiaoji drug-containing serum group reversed the above changes (P < 0.05 or P < 0.01)， and the combined intervention produced greater effects than either intervention alone (P < 0.05 or P < 0.01). Conclusion: Lifei Xiaoji Pills can reverse EMT in A549 cells and inhibit NSCLC invasion， and its mechanisms may be related to regulation of the lncRNA HOTAIR/Wnt/β-catenin pathway.
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基本信息:
DOI：10.13412/j.cnki.zyyl.20260928.005
中图分类号:R285
引用信息:
[1]刘素晓,李素云,杜瑞丽,等.理肺消积丸经lncRNA HOTAIR/Wnt/β-catenin通路逆转A549细胞上皮间质转化抑制NSCLC细胞侵袭[J].中药药理与临床().DOI:10.13412/j.cnki.zyyl.20260928.005.
基金信息:
河南省自然科学基金（编号：252300420612）; 河南省中医药科学研究专项课题（编号：2023ZY1006、2024ZY1004）
2026-09-29
2026-09-29
2026-09-29